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Methods: Identification and molecular characterization of the major melon allergen were performed using IgE immunoblotting, allergen-specific ELISA, affinity-based purifications, cross-inhibition assays, cloning, and expression of the allergen in Escherichia coli. Results: Melon profilin was identified and isolated as a major IgE-binding component and designated as Cuc m 2. Significant cross-reactivity was found between melon profilin and Cynodon dactylon, tomato, peach, and grape profilins in cross-inhibition assays.
Although the highest degree of amino acid identity was revealed with watermelon profilin, there was no significant cross-reactivity between melon and watermelon profilins. Conclusion: Melon profilin is the major IgE-binding component in melon extract, and the recombinant and natural forms exhibited similar IgE-binding capacities.
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A part of the fruit-fruit and pollen-fruit cross-reactions could be explained by the presence of this conserved protein; however, sequence homology provides insufficient information to predict IgE cross-reactivity of profilins. Many molecular techniques used to understand the maturation of these fruits require high concentrations of highly purified RNA.
However, melons are rich in polyphenolic compounds and polysaccharides, which interfere with RNA extraction. This study aimed to determine the most appropriate method for total RNA extraction from melon fruits. In general, higher amounts of RNA were obtained from green than mature fruits, probably due to the lower concentration of polysaccharides and water. Oct 30, Melon is an important dessert fruit in the Sistan region of.
Iran, but its cultivation is threatened by attacks of.
Melon death induced by these soil-borne plant pathogenic Endogenous microbial contamination of melons Cucumis melo from international trade: an underestimated risk for the consumer? Fruits and vegetables have increasingly been related to foodborne outbreaks. Besides surface contamination, a possible internalization of microorganisms into edible parts of plants during growth has already been observed.
One hundred percent of the rind samples [3. Among the pulp isolates, opportunistic and potentially pathogenic bacteria were identified, mainly Staphylococcus spp. Salmonella spp. Clostridium perfringens was isolated from the rind of seven melons. The present study revealed a regularly occurring internal contamination of melons.
Possible health risks for consumers because of an occurrence of microorganisms in melon pulp should be considered in future food safety assessments. Marker-assisted selection of Fusarium wilt-resistant and gynoecious melon Cucumis melo L.
Markers of A and F are cleaved amplified polymorphic sequences that distinguish alleles according to restriction analysis. Twenty F1 and F2 plants derived from the crosses between the gynoecious line WI and the Fusarium wilt-resistant line MR-1 were genotyped based on the markers. The results showed that the polymerase chain reaction and enzyme digestion results could be effectively used to identify plants with the AAggFF genotype in F2 populations.
In the F2 population, 35 gynoecious wilt-resistant plants were selected by marker-assisted selection and were confirmed by disease infection assays, demonstrating that these markers can be used in breeding to select F. Effects of gamma irradiation of pollen on parthenogenetic haploid production in muskmelon Cucumis melo L. G1 genotype. Regardless of doses of between 0. The pollen tube from pollen irradiated with up to 2. When pollen was cultivated in vitro, relatively high doses of irradiation 1. Radiation-induced changes in the generative nucleus led to the formation of two chromosomally unbalanced sperm cells as indicated by the appearance of morphological dimorphism which induced parthenogenetic development of the egg to form a haploid embryo.
Haploid embryo production by gamma-irradiated pollen was genotype dependent. For exposures of between 0. The ploidy of the plantlets in vitro was determined by flow cytometry. No aneuploidy was detected. All resulting plants exhibited normal phenotypes. Key words: Melon Plant Dis. The use of gamma radiation aiming to post harvest conservation of Cantaloupe melon Cucumis melo L. Cantaloupensis ;Utilizacao de radiacao gama em meloes cantaloupe Cucumis melo L.
Cantaloupensis como tecnica de conservacao pos-colheita. Although Brazilian fruit culture has been growing in the international market, the fruit quality and the post harvest technology have not been improved properly. In Brazil, fruit nutritional factors are very important because of their potential to provide suitable nutrients for a significant part of the Country population. Some post harvest technologies, such as ionizing radiation, can keep the physical, chemical, nutritional and sensorial characteristics of the natural fruit, improving the quality of the fruits in the market.
This work evaluated the effects of Cobalt 60 irradiation in Cantaloupe melon, aiming the post harvest conservation during 7 days of storage, at a temperature ranging from 20 to 22 deg C. The doses of irradiation were set to 0, , , , , and Gy, based on the multiple of Gy quarantine dose, aiming to establish the lowest, the highest and the ideal doses.
Afterwards, physical, chemical and nutritional characteristics of irradiated fruit were checked and, finally, the sensorial characteristics through acceptability test. The storage period was the most important factor that affected the quality of the fruit, despite of the radiation doses. Based on the acceptability test, the best evaluated fruits were from the treatments of and Gy. This work allowed to conclude that fruit radiation is an appropriate technology for Cantaloupe melon post harvest conservation, but it is necessary to be used in combination with other technologies, especially to fungi control.
Temas de Historia: El golpe de Melo. Medieval herbal iconography and lexicography of Cucumis cucumber and melon, Cucurbitaceae in the Occident, The genus Cucumis contains two species of important vegetable crops, C. Melon has iconographical and textual records from lands of the Mediterranean Basin dating back to antiquity, but cucumber does not.
The goal of this study was to obtain an improved understanding of the history of these crops in the Occident. Medieval images purportedly of Cucumis were examined, their specific identity was determined and they were compared for originality, accuracy and the lexicography of their captions. The manuscripts having accurate, informative images are derived from Italy and France and were produced between and All have an illustration of cucumber but not all contain an image of melon. The cucumber fruits are green, unevenly cylindrical with an approx.
Most of the images show the cucumbers marked by sparsely distributed, large dark dots, but images from northern France show them as having densely distributed, small black dots. The different size, colour and distribution reflect the different surface wartiness and spininess of modern American and French pickling cucumbers. The melon fruits are green, oval to serpentine, closely resembling the chate and snake vegetable melons, but not sweet melons. In nearly all manuscripts of Italian provenance, the cucumber image is labelled with the Latin caption citruli, or similar, plural diminuitive of citrus citron, Citrus medica.
However, in manuscripts of French provenance, the cucumber image is labelled cucumeres, which is derived from the classical Latin epithet cucumis for snake melon.
One image, from British Library ms. Sloane , has a caption that allows tracing of the word 'gherkin' back to languages of the geographical nativity of C. Medieval herbal iconography and lexicography of Cucumis cucumber and melon, Cucurbitaceae in the Occident, — Background The genus Cucumis contains two species of important vegetable crops, C.
Findings The manuscripts having accurate, informative images are derived from Italy and France and were produced between and A conserved ethylene biosynthesis enzyme leads to andromonoecy in two cucumis species. Cucumis sativus cucumber and Cucumis melo melon that have diverged over 40 My ago. The isolation of the genes for andromonoecy in Cucumis species provides a molecular basis for understanding how sexual systems arise and are maintained within and between species. Full Text Available Brazil produced , metric tons of melons in , principally in the Northeast region where one of the most important melon pathogens is the powdery mildew fungus Podosphaera xanthii.
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The disease is controlled mainly by incorporating single dominant resistance genes into commercial hybrids. In the current experiment, the quantitative trait loci QTL analysis was done by composite interval mapping method to detect QTLs in edge, central parts and fruit shape of melon.
The total length of linkage map was found to be In a total, we detected ten QTLs, in which one was major, while others were minor.
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Five QTLs were detected in the edge part of melon fruit and three QTLs were detected in central parts of melon and all were considered as Brix content. Two QTLs were related with fruit shape. Our present genetic and QTLs mapping would be proved useful in plant breeding programs for the improvement of economically important horticultural traits.